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Buses in Prague
http://www.prague-guide.co.uk/articles/19/1/Buses-in-Prague/Page1.html
By Libor Kapicka
 
If you’re staying in Prague then you probably won’t be using buses, unless you are staying in some obscure suburbs.

If you’re staying in Prague then you probably won’t be using buses, unless you are staying in some obscure suburbs. Night-time service is provided by bus numbers 502 to 514 and 601 to 603. Bus schedules are located at individual stops. Regular buses operate from 4.30 a.m. - 12 p.m.

If you are travelling outside Prague then a bus might be quite a good option, as they are usually cheaper than travelling by train. Most services are operated by CSAD. For Bus departure and arrival information dial Tel. 900919041 or Tel. 224 210 221 (for international departures). You can also visit online timetable with prices at timetable.svt.cz.

City Buses.
Buses (autobusy) mostly cover the outskirts of Prague. Daytime buses run from 4:30 a.m. till midnight in 6-8 min. intervals in peak hours, 10-20 min. intervals in the off hours, and 15-30 min. intervals on weekends. Night buses (numbers 501-512) run from 00:30 a.m. till 4:30 a.m. in a 40 min. intervals.

Main bus stations in Prague:
Most international connections terminate in Prague at the Florenc station. 
- Florenc - Praha 8, Krizikova (Metro lines B and C, Florenc)
  (from here you can get to the centre by metro line B in about 5 minutes)
- Na Knizeci - Praha 5, Nadrazni (Metro B-line, Andel)
- Holesovice - Praha 7, Partyzanska (Metro C-line, Nadrazi Holesovice)
- Roztyly - Praha 4, Rysaveho (Metro C-line, Roztyly)
- Cerny Most - Praha 9 (Metro B-line, Cerny Most)

Special wheelchair accessible buses:
Special bus lines 1 and 3 providing transport for disabled persons in wheelchairs connect the suburbs Cerny Most II, Jizní Mesto II, Nové Butovice and Repy with the centre of Prague (Metro station Florenc) where the change of lines is possible. These lines are in service during the working days only.